APC antibody (AA 1-433)

Catalog No. ABIN6935533

Product Details anti-APC Antibody

Target: APC (Adenomatous Polyposis Coli (APC))

Binding Specificity: AA 1-433

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

No Cross-Reactivity: Mouse (Murine)

Purification: Purified by Protein A/G

Immunogen: Recombinant fragment of human APC protein (around aa 1-433)

Clone: ALi 12-28

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: COLO 320DM whole cell lysate. HCT116 cells. Tonsil, placenta, breast or colon.

Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Western Blot (0.5-1.0 μg/mL), Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for APC

Target: APC (Adenomatous Polyposis Coli (APC))

Alternative Name: APC (APC Products)

Synonyms: dp2 antibody, dp3 antibody, fap antibody, fpc antibody, xapc antibody, dp2.5 antibody, AI047805 antibody, AU020952 antibody, AW124434 antibody, CC1 antibody, Min antibody, mAPC antibody, BTPS2 antibody, DP2 antibody, DP2.5 antibody, DP3 antibody, GS antibody, PPP1R46 antibody, RATAPC antibody, APC1 antibody, APC, WNT signaling pathway regulator antibody, adenomatous polyposis coli antibody, adenomatous polyposis coli protein, putative antibody, adenomatosis polyposis coli antibody, adenomatous polyposis coli L homeolog antibody, APC antibody, apc antibody, Smp_139190 antibody, Apc antibody, apc.L antibody

Background: The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2,843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS. APC has been found to be associated with structural components of intracellular junctions. Functions as a tumor suppressor, promoting rapid degradation of CTNNB1 and is a negative regulator of Wnt signaling. Also plays a role in HGF-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Molecular Weight: 66kDa

Gene ID: 324

UniProt: P25054

Pathways: WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization