MCAM antibody
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- Target See all MCAM Antibodies
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MCAM antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)
- Purification
- Purified by Protein A/G
- Immunogen
- Recombinant human CD146 protein
- Clone
- C146-634
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product MCAM Primary Antibody
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- Application Notes
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Positive Control: A-375, HUV-EC or HeLa cells. Tonsil or Melanoma (IHC).
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
- Alternative Name
- MCAM (MCAM Products)
- Synonyms
- CD146 antibody, MUC18 antibody, Muc18 antibody, 1-gicerin antibody, AV025631 antibody, CD149 antibody, s-endo antibody, s-gicerin antibody, cd146 antibody, gicerin antibody, id:ibd3512 antibody, mcam antibody, MCAM antibody, melanoma cell adhesion molecule antibody, melanoma cell adhesion molecule b antibody, MCAM antibody, Mcam antibody, mcamb antibody
- Background
- The human CD146 gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. CD146 is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease.
- Molecular Weight
- 130kDa
- Gene ID
- 4162
- UniProt
- P43121
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