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MCAM antibody

MCAM Reactivity: Human FACS, IHC, IF, StM Host: Mouse Monoclonal C146-634 unconjugated
Catalog No. ABIN6940010
  • Target See all MCAM Antibodies
    MCAM (Melanoma Cell Adhesion Molecule (MCAM))
    Reactivity
    • 119
    • 47
    • 46
    • 14
    • 14
    • 9
    • 7
    • 6
    • 5
    • 2
    Human
    Host
    • 92
    • 48
    • 1
    • 1
    • 1
    Mouse
    Clonality
    • 86
    • 57
    Monoclonal
    Conjugate
    • 72
    • 12
    • 11
    • 8
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MCAM antibody is un-conjugated
    Application
    • 87
    • 66
    • 60
    • 44
    • 18
    • 16
    • 14
    • 10
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Staining Methods (StM)
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant human CD146 protein
    Clone
    C146-634
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product MCAM Primary Antibody
  • Application Notes

    Positive Control: A-375, HUV-EC or HeLa cells. Tonsil or Melanoma (IHC).

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    MCAM (Melanoma Cell Adhesion Molecule (MCAM))
    Alternative Name
    MCAM (MCAM Products)
    Synonyms
    CD146 antibody, MUC18 antibody, Muc18 antibody, 1-gicerin antibody, AV025631 antibody, CD149 antibody, s-endo antibody, s-gicerin antibody, cd146 antibody, gicerin antibody, id:ibd3512 antibody, mcam antibody, MCAM antibody, melanoma cell adhesion molecule antibody, melanoma cell adhesion molecule b antibody, MCAM antibody, Mcam antibody, mcamb antibody
    Background
    The human CD146 gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18, that belongs to the immunoglobulin superfamily and functions as a Ca2+-independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. CD146 is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease.
    Molecular Weight
    130kDa
    Gene ID
    4162
    UniProt
    P43121
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