For best experience we recommend to activate Javascript in your browser.

Myc Tag antibody (C-Term)

Name: Myc Tag antibody (C-Term)
Brand: antibodies-online
SKU: ABIN6934473
Price: 285.08 USD
Availability: InStock

IF, IHC, FACS, StM Host: Mouse Monoclonal 9E10-3 unconjugated

Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with c-Myc Mouse Monoclonal Antibody (9E10. (Myc Tag antibody (C-Term))

IHC

SDS-PAGE Analysis Purified c-Myc Mouse Monoclonal Antibody (9E10. (Myc Tag antibody (C-Term))

SDS

2 images

Catalog No. ABIN6934473

Datasheet as PDF

Target See all Myc Tag products

Myc Tag
Binding Specificity
33

21

12

8

8

3

2

1

1
AA 408-439, C-Term

Reactivity

Please inquire
Host
83

48

10

9

7

7

1

1
Mouse
Compatible Secondaries
Clonality
94

60

10
Monoclonal
Conjugate
81

14

12

8

4

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This Myc Tag antibody is un-conjugated
Application
132

65

48

48

35

33

32

20

17

11

11

6

6

4

4

4

4

3

2

2

1

1

1

1

1

1
Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Specificity

It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.

No Cross-Reactivity

Chicken, Mouse (Murine), Rat (Rattus)

Purification

Purified by Protein A/G

Immunogen

A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc, coupled to KLH.

Clone

9E10-3

Isotype

IgG1 kappa
anti-Myc Tag antibody
WB, IP, ELISA, IF Host: Mouse Monoclonal unconjugated

anti-Myc Tag (C-Term) antibody
Reactivity: Human WB, IP, IHC (p), FACS Host: Mouse Monoclonal 9E10 unconjugated

anti-Myc Tag antibody
WB, IP, ELISA Host: Rabbit Polyclonal unconjugated

anti-Myc Tag (C-Term) antibody (FITC)
Reactivity: Human FACS Host: Mouse Monoclonal 9E10 FITC

anti-Myc Tag (C-Term) antibody (Biotin)
Reactivity: Human WB, IP, IHC (p), FACS Host: Mouse Monoclonal 9E10 Biotin

anti-Myc Tag antibody
WB, IP, ELISA, IF Host: Mouse Monoclonal 2G8D5 unconjugated

anti-Myc Tag (AA 408-439) antibody
WB, ELISA Host: Mouse Monoclonal 9E10- unconjugated

anti-Myc Tag antibody
WB, IP, IF Host: Mouse Monoclonal 3B8 unconjugated

anti-Myc Tag (AA 410-419) antibody
WB, IP, IF, IHC, ICC Host: Mouse Monoclonal Myc-A7 unconjugated

anti-Myc Tag antibody (Biotin)
WB, IP, ELISA, IHC Host: Rabbit Polyclonal Biotin

Application Notes

Positive Control: HL-60 cells. Cervical Carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions

For Research Use only
Concentration

200 μg/mL

Buffer

10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-80 °C

Storage Comment

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date

24 months
Target

Myc Tag

Abstract

Myc Tag Products

Target Type

Tag

Molecular Weight

62-64kDa

Gene ID

4609

UniProt

P01106