Myc Tag antibody (C-Term)
-
- Target See all Myc Tag products
- Myc Tag
-
Binding Specificity
- AA 408-439, C-Term
- Reactivity
- Please inquire
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This Myc Tag antibody is un-conjugated
-
Application
- Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)
- Specificity
- It recognizes a transcription factor of 64-67 kDa, identified as c-myc. Its epitope spans between aa 410-419 (EQKLISEEDL) which is a specific portion of an alpha helical region of human c-myc protein. This MAb shows no cross-reaction with v-myc. c-myc is involved in the control of cell proliferation and differentiation and is amplified and/or overexpressed in a variety of tumors. Over-expression of c-myc protein occurs frequently in luminal cells of prostate intraepithelial neoplasia as well as in most primary carcinomas and metastatic disease.
- No Cross-Reactivity
- Chicken, Mouse (Murine), Rat (Rattus)
- Purification
- Purified by Protein A/G
- Immunogen
- A synthetic peptide, corresponding to aa 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) from C-terminus of human c-myc, coupled to KLH.
- Clone
- 9E10-3
- Isotype
- IgG1 kappa
-
-
- Application Notes
-
Positive Control: HL-60 cells. Cervical Carcinoma.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
-
- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
-
- Target
- Myc Tag
- Abstract
- Myc Tag Products
- Target Type
- Tag
- Molecular Weight
- 62-64kDa
- Gene ID
- 4609
- UniProt
- P01106
-