CD34 antibody
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- Target See all CD34 Antibodies
- CD34
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Reactivity
- Human, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD34 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Coating (Coat)
- Specificity
- This antibody recognizes a carbohydrate epitope on a single chain, transmembrane, heavily glycosylated protein of 90-120 kDa, which is identified as CD34 (VI international workshop on human differentiation antigens). Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels 85 % of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
- Purification
- Purified by Protein A/G
- Immunogen
- Blast cells of a chronic myeloid leukemia patient
- Clone
- ICO-115
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD34 Primary Antibody
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- Application Notes
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Positive Control: KG-1 cells. Human tonsil or angiosarcoma.
Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Optimal dilution for a specific application should be determined.
- Restrictions
- For Research Use only
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- Concentration
- 200 μg/mL
- Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
- Expiry Date
- 24 months
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- Target
- CD34
- Alternative Name
- CD34 (CD34 Products)
- Synonyms
- AU040960 antibody, CD34 molecule antibody, CD34 antigen antibody, CD34 antibody, Cd34 antibody
- Molecular Weight
- 90-110kDa
- Gene ID
- 947
- UniProt
- P28906
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