E-cadherin antibody

Catalog No. ABIN6941296

The Mouse Monoclonal anti-E-cadherin antibody (Clone SPM381) (ABIN6941296) specifically detects E-cadherin in WB, IHC, FACS and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for E-cadherin antibody (ABIN6941296)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SPM381

Product Details anti-E-cadherin Antibody

Purpose: E-Cadherin/ CD324 (Intercellular Junction Marker) Antibody

Specificity: Recognizes a protein of 120-80 kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as æ-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human E-Cadherin protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Positive Control: LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.

Known Application: Immunofluoresence (2-4 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1

Background:

Arc 1, cadherin 1 type 1 E-cadherin, Cadherin1, CAM 120/80, CD324, CDHE, E-Cad/CTF3, ECAD, Epithelial cadherin, epithelial calcium dependent adhesion protein, Liver cell adhesion molecule (LCAM), Uvomorulin (UVO)

LS174T, Rajior SK-BR3 cells. Human prostate or colon carcinomas.

Molecular Weight: 120-80kDa (Mature), 135kDa (Precursor)

Gene ID: 999, 461086

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation