Keratin 5/8 antibody

Catalog No. ABIN6941336

Product Details anti-Keratin 5/8 Antibody

Target: Keratin 5/8 (KRT5/8)

Reactivity: Human, Mouse, Pig, Cow, Rat, Dog, Hamster, Sheep, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 5/8 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)

Specificity: It reacts with keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

No Cross-Reactivity: Chicken, Xenopus laevis

Purification: Purified by Protein A/G

Immunogen: Cytoskeletal preparation from HeLa cells

Clone: C-50

Isotype: IgG1 kappa

Application Details

Application Notes:

Positive Control: HepG2 or A431 cells. Skin or Squamous cell carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT)Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: 0.5 mL at 200 µg/mL of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10 mM PBS, pH 7.4 with 0.05 % BSA and 0.05 % sodium azide. Contact us if you require this antibody in a different format.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 to 8°C. Antibody is stable for 24 months.

Expiry Date: 24 months

Target Details for Keratin 5/8

Target: Keratin 5/8 (KRT5/8)

Alternative Name: KRT5 & KRT8 (KRT5/8 Products)

Molecular Weight: 58kDa (CK5), 52.5kDa (CK8)

Gene ID: 3852, 3856

UniProt: P13647, P05787