Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Keratin 77 antibody

KRT77 Reactivity: Human, Rat IHC, StM Host: Mouse Monoclonal KRTL-1077 unconjugated
Catalog No. ABIN6941447
  • Target See all Keratin 77 (KRT77) Antibodies
    Keratin 77 (KRT77)
    Reactivity
    • 25
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat
    Host
    • 18
    • 7
    Mouse
    Clonality
    • 16
    • 9
    Monoclonal
    Conjugate
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Keratin 77 antibody is un-conjugated
    Application
    • 11
    • 9
    • 6
    • 6
    • 5
    • 3
    • 2
    Immunohistochemistry (IHC), Staining Methods (StM)
    Specificity
    This MAb recognizes the 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40 kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
    Cross-Reactivity (Details)
    Shows broad species reactivity.
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant human KRT77 protein
    Clone
    KRTL-1077
    Isotype
    IgG1 kappa
  • Application Notes

    Positive Control: Skin, Squamous cell carcinoma (SCC).

    Known Application: Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    Keratin 77 (KRT77)
    Alternative Name
    KRT77 (KRT77 Products)
    Synonyms
    K1B antibody, KRT1B antibody, 4732484G22Rik antibody, K1emb antibody, Krt239 antibody, mK1b antibody, Kb39 antibody, keratin 77 antibody, KRT77 antibody, Krt77 antibody
    Molecular Weight
    56.5kDa (CK10), 50kDa (CK14), 50kDa (CK15), 48kDa (CK16), 40kDa (CK19)
    Gene ID
    374454
    UniProt
    Q7Z794
You are here:
Support