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Recombinant KRT77, KRT76 antibody

Reactivity: Human IHC, StM Host: Rabbit Monoclonal KRTH-1576R-KRTL-1577R unconjugated Recombinant Antibody
Catalog No. ABIN6941457
  • Target
    KRT77, KRT76
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 8
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    Human
    Host
    • 6
    • 2
    Rabbit
    Clonality
    • 7
    • 1
    Monoclonal
    Conjugate
    • 6
    • 1
    • 1
    Un-conjugated
    Application
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    Immunohistochemistry (IHC), Staining Methods (StM)
    Specificity
    Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. It is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It may be useful to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has high sensitivity in the recognition of epithelial cells and carcinomas.
    Cross-Reactivity (Details)
    Expected to show broad species reactivity.
    Purification
    Purified by Protein A/G
    Immunogen
    Recombinant full-length human KRT76 and KRT77 proteins
    Clone
    KRTH-1576R-KRTL-1577R
    Isotype
    IgG
  • Application Notes

    Positive Control: Skin, Adeno- or Squamous carcinomas.

    Known Application: Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Restrictions
    For Research Use only
  • Concentration
    200 μg/mL
    Buffer
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-80 °C
    Storage Comment
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    Expiry Date
    24 months
  • Target
    KRT77, KRT76
    Alternative Name
    KRT77 & KRT76
    Molecular Weight
    40-67kDa
    Gene ID
    374454, 51350
    UniProt
    Q7Z794, Q01546
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