CBFB antibody

Catalog No. ABIN6942372

Product Details anti-CBFB Antibody

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CBFB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Cross-Reactivity: Human, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant protein within human CBFb aa 1-200.

Clone: 5D4

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for CBFB

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Alternative Name: CBFB (CBFB Products)

Synonyms: PEBP2B antibody, Cbfb antibody, pebp2b antibody, Pebp2 antibody, CBFB antibody, unp1266 antibody, cbf-beta antibody, cbfbeta antibody, pea2-beta antibody, pebp2-beta antibody, AI893578 antibody, PEA2 antibody, PEBP2b antibody, Pebpb2 antibody, core-binding factor beta subunit antibody, core binding factor beta subunit antibody, core-binding factor, beta subunit antibody, core-binding factor, beta subunit S homeolog antibody, core binding factor beta antibody, CBFB antibody, Cbfb antibody, cbfb antibody, cbfb.S antibody

Background:

Synonyms: Core-binding factor subunit beta, Polyomavirus enhancer-binding protein 2 beta subunit, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit, CBF-beta, PEA2-beta, PEBP2-beta, CBFB

Background: Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation.

Gene ID: 865

UniProt: Q13951

Pathways: Regulation of Lipid Metabolism by PPARalpha