PCNA antibody (Proliferating Cell Nuclear Antigen)

Details for Product anti-PCNA Antibody No. ABIN6944453
Antigen
  • PCNAR
  • Pcna/cyclin
  • pcna
  • AO090003000846
  • 53/13
  • CG9193
  • DmPCNA
  • Dmel\\CG9193
  • MUS209
  • Mus209
  • PCNA
  • PCNA/mus209
  • Pcna
  • dPCNA
  • l(2)02448
  • l(2)56F
  • l(2)56Fa
  • l(2)s1534
  • cb16
  • fb36g03
  • wu:fa28e03
  • wu:fb36g03
  • pcna-A
  • proliferating cell nuclear antigen
  • DNA polymerase III sliding clamp
  • Proliferating cell nuclear antigen
  • Proliferating cell nuclear antigen-like
  • proliferating cell nuclear antigen L homeolog
  • proliferating cell nuclear antigen 1
  • PCNA
  • Pcna
  • pcna
  • MMP_RS08810
  • ANI_1_512014
  • AOR_1_1500154
  • MGC53867
  • pcna.L
  • pcna1
  • LOC106420145
  • pcn1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
506
270
254
62
51
45
43
38
37
26
22
18
12
12
11
8
7
5
5
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
303
234
4
2
Clonality (Clone)
Monoclonal ()
Conjugate
This PCNA antibody is un-conjugated
24
20
11
8
8
8
8
8
8
6
4
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (IHC), Western Blotting (WB)
402
190
120
120
118
103
78
53
44
34
21
11
10
7
7
4
3
2
1
1
1
1
1
Options
Immunogen Recombinant human PCNA between 100 to 200 amino acids
Clone 1G3
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others PCNA products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name PCNA (PCNA Antibody Abstract)
Background

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.


Synonyms: ProlifeRating cell nuclear antigen, Cyclin, PCNA
Gene ID 5111
UniProt P12004
Pathways Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
Application Notes WB(1:300-1000), FCM(1:20-100), IF(ICC)(1:50-200), IHC()
Restrictions For Research Use only
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Expiry Date 12 months
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