PIM1 antibody (Pim-1 Oncogene)

Details for Product anti-PIM1 Antibody No. ABIN6944550
Antigen
  • PIM
  • Pim-1
  • PIM1
  • pim
  • pim-1
  • pim3
  • Pim-1 proto-oncogene, serine/threonine kinase
  • proviral integration site 1
  • Pim-1 proto-oncogene, serine/threonine kinase L homeolog
  • PIM1
  • Pim1
  • pim1
  • pim1.L
Reactivity
Human, Mouse (Murine)
195
87
71
6
6
5
5
5
3
3
2
2
1
1
Host
Rabbit
158
54
1
Clonality (Clone)
Monoclonal ()
Conjugate
This PIM1 antibody is un-conjugated
18
6
6
4
4
4
4
4
4
4
4
4
1
1
1
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Western Blotting (WB)
140
105
57
35
34
13
10
10
7
7
2
1
1
Options
Immunogen Human PIM1 between 1-100 amino acids
Clone 5A12
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others PIM1 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name PIM1 (PIM1 Antibody Abstract)
Background

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity. The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Phosphorylation of MAP3K5, an other proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis. Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C. Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability. Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels. Phosphorylation of CDKN1B,induces 14-3-3-proteins binding, nuclear export and proteasome-dependent degradation. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis.


Synonyms: Serine/threonine-protein kinase pim-1, PIM1
Gene ID 5292
UniProt P11309
Pathways Glycosaminoglycan Metabolic Process
Application Notes WB(1:300-1000), IF(ICC)(1:50-200)
Restrictions For Research Use only
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Expiry Date 12 months
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