Recombinant mEos antibody (Alexa Fluor 647)
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- Target
- mEos
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
- Reactivity
- Lobophyllia hemprichii
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Host
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Alpaca
- Expression System
- E.coli
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Clonality
- Monoclonal
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Conjugate
- Alexa Fluor 647
- Application
- Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- Camelid sdAb anti-mEOS conjugated with Alexa647, Clone 1E8
- Specificity
- Recognizes mEOS and most common mEOS derivatives
- Characteristics
- A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.
- Purification
- Produced in: E.coli
- Clone
- 1E8
- Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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anti-mEos antibody (Atto 488)
Reactivity: Lobophyllia hemprichii ICC, IF, FACS Host: Alpaca Monoclonal 1E8 Atto 488 single-domain Antibody (sdAb) Recombinant Antibody
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- Application Notes
- Recommended dilution 1:500
- Comment
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Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- 2.5 μM fluorescently labeled sdAb in buffered saline, 50 % glycerol, 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Protect from light!
- Storage
- -20 °C,-80 °C
- Storage Comment
- Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below
- Expiry Date
- 12 months
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- Target
- mEos
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