CD8 alpha antibody (FITC)
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- Target See all CD8 alpha (CD8A) Antibodies
- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD8 alpha antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS)
- Purification
- This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze.
- Clone
- OKT8
- Isotype
- IgG2a
- Top Product
- Discover our top product CD8A Primary Antibody
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- Application Notes
- This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 μLper test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
- Comment
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5 uL (0.125 ug)/test
- Restrictions
- For Research Use only
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- Buffer
- 10 mM NaH2PO4, 150 mM NaCl, 0.09 % Sodium azide, 0.1 % gelatin, pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- 2-8°C protected from light
- Expiry Date
- 12 months
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- Target
- CD8 alpha (CD8A) (CD8a Molecule (CD8A))
- Alternative Name
- CD8a (CD8A Products)
- Synonyms
- cd8 antibody, LOC100136450 antibody, BB154331 antibody, Ly-2 antibody, Ly-35 antibody, Ly-B antibody, Lyt-2 antibody, zgc:136643 antibody, CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, RHACD8-4 antibody, CD8a molecule antibody, uncharacterized LOC100125537 antibody, CD8 alpha antibody, CD8 antigen, alpha chain antibody, T-cell surface glycoprotein CD8 alpha chain antibody, Cd8a antibody, LOC100125537 antibody, LOC100136450 antibody, CD8A antibody, cd8a antibody, LOC100126556 antibody
- Background
- The OKT8 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen- bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracel- lular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites. The OKT8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. If used together with alternative antibodies Anti-Human CD8a clone RPA-T8 or Anti-Human CD8a clone Hit8a, the OKT8 antibody will not block binding of RPA-T8 or Hit8a.
- Gene ID
- 925
- UniProt
- P01732
- Pathways
- TCR Signaling
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