SREBF1 antibody (AA 301-407)

Catalog No. ABIN6972789

Product Details anti-SREBF1 Antibody

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Binding Specificity: AA 301-407

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SREBF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP), Supershift Assay (SSA)

Characteristics: SREBP-1 and SREBP-2 (Sterol regulatory element-binding protein 1 and 2) are members of the basic-helix-loop-helix-leucine zipper (bHLH-ZIP) family of transcription factors and are involved in the regulation of cholesterol homeostasis. SREBP-1 and SREBP-2 are normally membrane bound at the ER and nuclear envelope, but in sterol-depleted cells, the membrane-bound precursors are cleaved to generate a soluble isoforms that translocate to the nucleus. These active forms, which include the bHLH-ZIP domain, activate transcription of genes involved in the uptake and synthesis of cholesterol. SREBP-1 antibody (mAb) (Clone IgG-2A4) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, Immunoprecipitation, Supershift and Western blot, it has been shown to react with Human samples.

Purification: Protein A Chromatography

Immunogen: This SREBP-1 antibody was raised against a recombinant protein corresponding to amino acids 301-407 of human SREBP-1.

Clone: IgG-2A4

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for SREBF1

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Alternative Name: SREBP-1 (SREBF1 Products)

Synonyms: SREBP-1c antibody, SREBP1 antibody, bHLHd1 antibody, ADD-1 antibody, ADD1 antibody, D630008H06 antibody, SREBP-1 antibody, SREBP-1a antibody, SREBP1c antibody, Srebp1 antibody, SCREBP1 antibody, SREBP-1C antibody, SREBF1 antibody, srebp1 antibody, sterol regulatory element binding transcription factor 1 antibody, sterol regulatory element binding transcription factor 1 L homeolog antibody, sterol regulatory element binding protein-1 antibody, Sterol regulatory element Binding Protein antibody, SREBF1 antibody, Srebf1 antibody, srebf1.L antibody, SREBP-1 antibody, srebf1 antibody, sbp-1 antibody

Molecular Weight: 68 and 125 kDa

NCBI Accession: NP_001005291

Pathways: AMPK Signaling, Caspase Cascade in Apoptosis, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha