SREBF2 antibody (AA 833-1141)

Catalog No. ABIN6972791

Product Details anti-SREBF2 Antibody

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Binding Specificity: AA 833-1141

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SREBF2 antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: SREBP-1 and SREBP-2 (Sterol regulatory element-binding protein 1 and 2) are members of the basic-helix-loop-helix-leucine zipper (bHLH-ZIP) family of transcription factors and are involved in the regulation of cholesterol homeostasis. SREBP-1 and SREBP-2 are normally membrane bound at the ER and nuclear envelope, but in sterol-depleted cells, the membrane-bound precursors are cleaved to generate a soluble isoforms that translocate to the nucleus. These active forms, which include the bHLH-ZIP domain, activate transcription of genes involved in the uptake and synthesis of cholesterol. SREBP-2 antibody (mAb) (Clone IgG-1C6) was raised in a Mouse host. It has been validated for use in Western blot, it has been shown to react with Human samples.

Purification: Protein G Chromatography

Immunogen: This SREBP-2 antibody was raised against a recombinant protein corresponding to amino acids 833-1141 of human SREBP-2.

Clone: IgG-1C6

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: Purified IgG in 70 mM Tris ( pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30 % glycerol and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Target Details for SREBF2

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Alternative Name: SREBP-2 (SREBF2 Products)

Synonyms: SREBF2 antibody, srebp2 antibody, SREBP2 antibody, zgc:158371 antibody, AI608257 antibody, SREBP-2 antibody, SREBP2gc antibody, bHLHd2 antibody, lop13 antibody, nuc antibody, Srebf2_retired antibody, sterol regulatory element binding transcription factor 2 antibody, sterol regulatory element binding factor 2 antibody, sterol regulatory element binding transcription factor 2 L homeolog antibody, SREBF2 antibody, srebf2 antibody, Srebf2 antibody, srebf2.L antibody

Molecular Weight: 68 and 125 kDa

NCBI Accession: NP_004590

Pathways: Regulation of Lipid Metabolism by PPARalpha