STAT3 antibody (pTyr705) (Cy3)

Catalog No. ABIN6984004

This Mouse Monoclonal antibody specifically detects STAT3 in IF (cc). It exhibits reactivity toward Human.

Quick Overview for STAT3 antibody (pTyr705) (Cy3) (ABIN6984004)

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT3 antibody is conjugated to Cy3

Application: Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 3F10

Product Details anti-STAT3 Antibody (Cy3)

Binding Specificity: pTyr705

Cross-Reactivity: Human

Purification: Purified by Protein G.

Immunogen: KLH conjugated Synthesised phosphopeptide derived from human STAT3 around the phosphorylation site of Tyr705

Isotype: IgG

Application Details

Application Notes: IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for STAT3

Target: STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))

Alternative Name: STAT3

Background:

Synonyms: STAT3 (phospho Y705), p-STAT3 (phospho Y705), Phospho-Stat3_pTyr705? STAT3(Phospho-Tyr705), p-STAT3(Tyr705), Phosphorylated Stat3(pTyr705), p-Stat3, Acute Phase Response Factor, APRF, DNA binding protein APRF, FLJ20882, MGC16063, Signal Transductor and Activator of Transcription 3, STAT 3, STAT3_HUMAN.

Background: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. Gene Symbol: APRF, FLJ20882, HIES, MGC16063.

Gene ID: 6774

UniProt: P40763

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor