IKK alpha antibody (C-Term)

Catalog No. ABIN6990273

This anti-IKK alpha antibody is a Rabbit Polyclonal antibody detecting IKK alpha in WB, ELISA, IF and ICC. Suitable for Human.

Quick Overview for IKK alpha antibody (C-Term) (ABIN6990273)

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKK alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-IKK alpha Antibody

Binding Specificity: C-Term

Specificity: Antibody has no cross response to IKKb or IKKg.

Purification: IKK alpha Antibody is affinity chromatography purified via peptide column.

Immunogen: IKK alpha antibody was raised against a 19 amino acid peptide near the carboxy terminus of human IKK alpha. The immunogen is located within the last 50 amino acids of IKK alpha.

Isotype: IgG

Application Details

Application Notes: IKK alpha can be used for detection of IKK alpha by Western blot at 1 μ,g/mL. An 85 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: IKK alpha Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: IKK alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for IKK alpha

Target: IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))

Alternative Name: IKK alpha

Background: IKK alpha Antibody: Nuclear factor kappa B (NF-κ,B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κ,B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFa, and bacteria product LPS. NF-κ,B is associated with Iκ,B proteins in the cell cytoplasm, which inhibit NF-κ,B activity. The long-sought Iκ,B kinase (IKK), which phosphorylates Iκ,B, and mediates Iκ,B degradation and NF-κ,B activation, was recently identified by several laboratories. IKK is a serine protein kinase, and the IKK complex contains alpha and beta subunits (IKKα, and IKKβ,). IKKα, and IKKβ, interact with each other and both are essential for the NF-κ,B activation. IKKα, specifically phosphorylates IkB-alpha. IKKα, is expressed in variety of human tissues.

Molecular Weight: 85 kDa

Gene ID: 1147

UniProt: O15111

Pathways: PI3K-Akt Signaling, NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins