BNIP3L/NIX antibody (AA 60-110)

Catalog No. ABIN6990342

Product Details anti-BNIP3L/NIX Antibody

Target: BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))

Binding Specificity: AA 60-110

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP3L/NIX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: At least two isoforms of Bnip3L are known to exist.

Purification: Bnip3L Antibody is affinity chromatography purified via peptide column.

Immunogen: Bnip3L antibody was raised against a 16 amino acid peptide near the center of human Bnip3L. The immunogen is located within amino acids 60 - 110 of Bnip3L.

Isotype: IgG

Application Details

Application Notes: Bnip3L antibody can be used for detection of Bnip3L by Western blot at 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Bnip3L Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Bnip3L antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for BNIP3L/NIX

Target: BNIP3L/NIX (BNIP3L) (BCL2/adenovirus E1B 19kDa Interacting Protein 3-Like (BNIP3L))

Alternative Name: Bnip3L (BNIP3L Products)

Synonyms: BNIP3a antibody, NIX antibody, NIP3 antibody, Nix antibody, Nip3 antibody, C86132 antibody, D14Ertd719e antibody, Nip3L antibody, bnip3l antibody, nip3 antibody, zgc:110478 antibody, bnip3l2 antibody, wu:fb52b03 antibody, wu:fc02h04 antibody, BCL2 interacting protein 3 like antibody, BCL2 interacting protein 3 antibody, BCL2/adenovirus E1B interacting protein 3 antibody, BCL2/adenovirus E1B interacting protein 3-like antibody, BCL2/adenovirus E1B interacting protein 3-like b antibody, BCL2/adenovirus E1B interacting protein 3-like a antibody, BNIP3L antibody, BNIP3 antibody, Bnip3l antibody, Bnip3 antibody, bnip3lb antibody, bnip3la antibody

Background: Bnip3L Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3alpha, and Nix (for Nip3-like protein X). Bnip3L/Bnip3alpha/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues. Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.

Molecular Weight: Predicted: 24 kDa

Observed: 36, 40 kDa

Gene ID: 665

NCBI Accession: NP_004322

UniProt: O60238

Pathways: Autophagy