NOD2 antibody (N-Term)

Catalog No. ABIN6990388

Product Details anti-NOD2 Antibody

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: At least four isoforms of NOD2 are known to exist, this antibody will only detect the longest isoform. NOD2 has no cross-reaction with NOD1.

Purification: NOD2 Antibody is immunoaffinity chromatography purified IgG.

Immunogen: NOD2 antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino terminus of human NOD2. The immunogen is located within the first 50 amino acids of NOD2.

Isotype: IgG

Application Details

Application Notes: NOD2 antibody can be used for detection of NOD2 by Western blot at 1 to 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 10 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NOD2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NOD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for NOD2

Target: NOD2 (Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2))

Alternative Name: NOD2 (NOD2 Products)

Synonyms: ACUG antibody, BLAU antibody, CARD15 antibody, CD antibody, CLR16.3 antibody, IBD1 antibody, NLRC2 antibody, NOD2B antibody, PSORAS1 antibody, Card15 antibody, F830032C23Rik antibody, Nlrc2 antibody, caspase recruitment domain 15 protein antibody, nucleotide binding oligomerization domain containing 2 antibody, nucleotide-binding oligomerization domain containing 2 antibody, TTHERM_01951320 antibody, NOD2 antibody, Nod2 antibody

Background: NOD2 Antibody: Apaf-1 and NOD1 are members of a new family, which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2. NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-κ,B, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn's disease that is a main type of chronic inflammatory bowel disease.

Molecular Weight: Predicted: 114 kDa

Observed: 100 kDa

Gene ID: 64127

UniProt: Q9HC29

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome