CARD8 antibody (C-Term)

Catalog No. ABIN6990435

Product Details anti-CARD8 Antibody

Target: CARD8 (Caspase Recruitment Domain Family, Member 8 (CARD8))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CARD8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: CARD8 Antibody is affinity chromatography purified via peptide column.

Immunogen: CARD8 antibody was raised against a synthetic peptide corresponding to amino acids at the C-terminus of human CARD8. The immunogen is located within the last 50 amino acids of CARD8.

Isotype: IgG

Application Details

Application Notes: CARD8 antibody can be used for detection of CARD8 by Western blot at 1 -2 μ,g/mL. An approximate 45 kDa can be detected. Antibody can also be used for immunocytochemistry starting at 10 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CARD8 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CARD8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for CARD8

Target: CARD8 (Caspase Recruitment Domain Family, Member 8 (CARD8))

Alternative Name: CARD8 (CARD8 Products)

Synonyms: CARD8 antibody, CARDINAL antibody, DACAR antibody, DAKAR antibody, NDPP antibody, NDPP1 antibody, TUCAN antibody, caspase recruitment domain family, member 8 antibody, caspase recruitment domain-containing protein 8 antibody, caspase recruitment domain family member 8 antibody, CARD8 antibody, LOC718594 antibody

Background: CARD8 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, BCL-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD8. This protein interacts with DRAL, a p53-responsive protein implicated in the induction of apoptosis, and caspase-1 and its related proteins ICEBERG and pesudo-ICE. Although there are conflicting reports on whether CARD8 acts a pro- or anti-apoptotic protein, it has been suggested that it functions as an adaptor molecule regulating caspase-1 and NF-κ,B activation.

Molecular Weight: Predicted: 47 kDa

Observed: 47 kDa

Gene ID: 22900

UniProt: Q9Y2G2

Pathways: Positive Regulation of Endopeptidase Activity