Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)
Purification
MYD88 Antibody is affinity chromatography purified via peptide column.
Immunogen
Anti-MYD88 antibody (2127) was raised against a peptide corresponding to 17 amino acids near carboxy terminus of human MYD88 isoform 1. The immunogen is located within the last 50 amino acids of MYD88.
MYD88
Reactivity: Human
WB, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 2 μ,g/mL, IF: 20 μ,g/mL.
Antibody validated: Western Blot in human, mouse and rat samples, Immunofluorescence and Immunoprecipitation in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
MYD88 Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
MYD88 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
MYD88, myeloid differentiation primary response 88, was identified as an innate immune signal transduction adaptor involved in the Toll-like receptor (TLR) and interleukin-1 (IL-1) signaling pathway (1,2,3) and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 functions as an adaptor protein for TLRs and IL-1 receptors, which stimulates IRAKs, IRF7 and TRAF6, leading to NF-?B activation, cytokine secretion and inflammatory response (2, 4,5,6). Nuclear factor-kappa-B activation modulates multiple genes regulating the body's immune reactions and inflammatory responses. MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-?B activation(4,5). MyD88 is also employed as a regulator molecule by IL-18 receptor. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs TLR2 and TLR4 as the signaling receptors(7,8). MyD88 gene is expressed in many tissues.