Smac Antibody is affinity chromatography purified via peptide column.
Immunogen
Anti-Smac antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human Smac. The immunogen is located within the last 50 amino acids of Smac.
DIABLO
Reactivity: Human
ELISA, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1 μ,g/mL, IHC: 5 μ,g/mL.
Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Smac Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
Smac antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
DIABLO
(Second Mitochondria-Derived Activator of Caspase (DIABLO))
Smac Antibody: The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO. Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.