Nephrin Antibody is affinity chromatography purified via peptide column.
Immunogen
Anti-Nephrin antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin.
Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in mouse and rat samples, Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Nephrin Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
Nephrin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
Nephrin (NPHS1)
(Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))
Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.