Reticulon 4 antibody (AA 200-250)

Catalog No. ABIN6990736

Product Details anti-Reticulon 4 Antibody

Target: Reticulon 4 (RTN4)

Binding Specificity: AA 200-250

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Reticulon 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: At least five isoforms of Nogo are known to exist, this antibody is specific for NogoA and NogoE.

Purification: NogoA Antibody is affinity chromatography purified via peptide column.

Immunogen: NogoA antibody was raised against a 19 amino acid synthetic peptide from near the center of human NogoA. The immunogen is located within amino acids 200 - 250 of NogoA.

Isotype: IgG

Application Details

Application Notes: NogoA antibody can be used for detection of NogoA by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NogoA Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NogoA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for Reticulon 4

Target: Reticulon 4 (RTN4)

Alternative Name: NogoA (RTN4 Products)

Synonyms: ASY antibody, NI220/250 antibody, NOGO antibody, NOGO-A antibody, NOGOC antibody, NSP antibody, NSP-CL antibody, Nbla00271 antibody, Nbla10545 antibody, Nogo-B antibody, Nogo-C antibody, RTN-X antibody, RTN4-A antibody, RTN4-B1 antibody, RTN4-B2 antibody, RTN4-C antibody, RTN4-Cw antibody, DKFZp459C0314 antibody, 1110020G17Rik antibody, AA407876 antibody, AA409940 antibody, AA960376 antibody, C130026I10Rik antibody, NgA antibody, Nogo-A antibody, mKIAA0886 antibody, mKIAA4153 antibody, NI-250 antibody, Nogo antibody, Vp20 antibody, r antibody, rat N antibody, rat NogoA antibody, reticulon-4 antibody, nogo antibody, rtn4 antibody, wu:fb17a02 antibody, wu:fb22f12 antibody, wu:fd61a07 antibody, zgc:92163 antibody, reticulon 4 antibody, reticulon 4a antibody, RTN4 antibody, rtn4 antibody, Rtn4 antibody, rtn4a antibody

Background: NogoA Antibody: NogoA is a member of a family of integral membrane proteins termed reticulons that are thought to be involved in numerous disorders including neurodegenerative diseases. Reticulon proteins are known to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor. It is highly expressed in oligodendrocytes in the white matter of the CNS, blocking its activity with antibodies or other factors results in improved axon regrowth and functional recovery in experimental CNS lesion models. NogoA has also been suggested to play a role in neurodegenerative diseases such as Amyotrophic lateral sclerosis, in which case NogoA is found at elevated levels in postmortem muscular samples, and multiple sclerosis (MS), in which case autoantibodies to NogoA have been found in serum and cerebrospinal fluid in MS patients.

Molecular Weight: Predicted: 131 kDa

Observed: ~180 kDa

Gene ID: 57142

NCBI Accession: NP_065393

UniProt: Q9NQC3

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome