MARCH8 antibody (C-Term)

Catalog No. ABIN6991011

Product Details anti-MARCH8 Antibody

Target: MARCH8 (Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Purification: MARCH8 Antibody is affinity chromatography purified via peptide column.

Immunogen: MARCH8 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MARCH8. The immunogen is located within the last 50 amino acids of MARCH8.

Isotype: IgG

Application Details

Application Notes: MARCH8 antibody can be used for detection of MARCH8 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MARCH8 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MARCH8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for MARCH8

Target: MARCH8 (Membrane-Associated Ring Finger (C3HC4) 8, E3 Ubiquitin Protein Ligase (MARCH8))

Alternative Name: MARCH8 (MARCH8 Products)

Synonyms: MARCH-VIII antibody, MIR antibody, RNF178 antibody, c-MIR antibody, zgc:171495 antibody, 1300017E09Rik antibody, Mir antibody, Mar-08 antibody, MARCH8 antibody, membrane associated ring-CH-type finger 8 antibody, membrane-associated ring finger (C3HC4) 8 antibody, membrane associated ring-CH-type finger 8 L homeolog antibody, MARCH8 antibody, march8 antibody, March8 antibody, march8.L antibody

Background: MARCH8 Antibody: MARCH8 (c-MIR) is a novel E3 ubiquitin ligase designated as the modulator of immune recognition (MIR) family, whose catalytic domain is a variant RING domain (RING-CH domain). MARCH8 was found as a functional and structural homolog of KSHV MIR1 and MIR2. MARCH8 targets B7-2 to lysosomal degradation and down-regulates the B7-2 surface expression through ubiquitination of its cytoplasmic tail. Furthermore, MARCH8 has been shown to down-regulate the expression of transferrin receptor and Fas, an important molecule for the induction of apoptosis. MARCH8 is the first example of an E3 ubiquitin ligase that is capable of inhibiting MHC II expression. Recent findings suggest that MARCH8 may regulate immune responses by promoting ubiquitination of MHC-II and CD86, leading to their subsequent endocytosis and lysosomal degradation.

Gene ID: 220972

NCBI Accession: NP_659458

UniProt: Q5T0T0