SIP1 antibody (C-Term)

Catalog No. ABIN6991364

Product Details anti-SIP1 Antibody

Target: SIP1 (GEMIN2) (Gem (Nuclear Organelle) Associated Protein 2 (GEMIN2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: SIP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: SIP1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human SIP1. The immunogen is located within the last 50 amino acids of SIP1.

Isotype: IgG

Application Details

Application Notes: SIP1 antibody can be used for detection of SIP1 by Western blot at 0.5 - 1 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 4 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SIP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SIP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SIP1

Target: SIP1 (GEMIN2) (Gem (Nuclear Organelle) Associated Protein 2 (GEMIN2))

Alternative Name: SIP1 (GEMIN2 Products)

Synonyms: SIP1 antibody, SIP1-delta antibody, 1700012N19Rik antibody, Sip1 antibody, sip1 antibody, sip1-delta antibody, wu:fc52a05 antibody, zgc:110274 antibody, gem nuclear organelle associated protein 2 antibody, gem (nuclear organelle) associated protein 2 antibody, gem nuclear organelle associated protein 2 S homeolog antibody, GEMIN2 antibody, Gemin2 antibody, gemin2.S antibody, gemin2 antibody

Background: SIP1 Antibody: SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. SIP1 interacts directly with the SMN and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of the SIP1 mRNA in motor neurons showed normal motor axons while that of SMN mRNA did show abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside of the SMN complex.

Gene ID: 8487

UniProt: O14893

Pathways: Ribonucleoprotein Complex Subunit Organization, Tube Formation