SDHD antibody (AA 30-80)

Catalog No. ABIN6991691

Product Details anti-SDHD Antibody

Target: SDHD (Succinate Dehydrogenase Complex, Subunit D, Integral Membrane Protein (SDHD))

Binding Specificity: AA 30-80

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SDHD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: At least four isoforms of SDHD are known to exist, this antibody will detect the two longest isoforms.

Purification: SDHD Antibody is affinity chromatography purified via peptide column.

Immunogen: SDHD antibody was raised against a 15 amino acid synthetic peptide near the center of human SDHD. The immunogen is located within amino acids 30 - 80 of SDHD.

Isotype: IgG

Application Details

Application Notes: SDHD antibody can be used for detection of SDHD by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μ,g/mL. For immunofluorescence start at 2.5 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: SDHD Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: SDHD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for SDHD

Target: SDHD (Succinate Dehydrogenase Complex, Subunit D, Integral Membrane Protein (SDHD))

Alternative Name: SDHD (SDHD Products)

Synonyms: RATSDHE1 antibody, SDH2 antibody, Sdh antibody, Sdhe1 antibody, TDH antibody, 3110001M13Rik antibody, AVLL5809 antibody, C78570 antibody, PRO19626 antibody, CII-4 antibody, CII4 antibody, QPs3 antibody, cybS antibody, CybS antibody, CBT1 antibody, CWS3 antibody, PGL antibody, PGL1 antibody, SDH4 antibody, MSN2.16 antibody, MSN2_16 antibody, succinate dehydrogenase 1-1 antibody, CybS-B antibody, cbt1 antibody, pgl antibody, pgl1 antibody, sdh4 antibody, cybS-B antibody, sdhd antibody, sdhda antibody, zgc:100986 antibody, serine dehydratase antibody, succinate dehydrogenase complex, subunit D, integral membrane protein antibody, succinate dehydrogenase complex subunit D antibody, succinate dehydrogenase 1-1 antibody, succinate dehydrogenase complex subunit D, integral membrane protein L homeolog antibody, succinate dehydrogenase complex, subunit D, integral membrane protein b antibody, Sds antibody, Sdhd antibody, SDHD antibody, SDH1-1 antibody, sdhd.L antibody, sdhdb antibody

Background: SDHD Antibody: The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH). Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.

Gene ID: 9392

NCBI Accession: NP_002993

UniProt: O14521