CRB2 antibody (AA 460-510)

Catalog No. ABIN6991796

Product Details anti-CRB2 Antibody

Target: CRB2 (Crumbs Homolog 2 (CRB2))

Binding Specificity: AA 460-510

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CRB2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Specificity: At least three isoforms of CRB2 are known to exist, this antibody will detect all three. CRB2 antibody is predicted to not cross-react with other members of the CRB family.

Purification: CRB2 Antibody is affinity chromatography purified via peptide column.

Immunogen: Rabbit polyclonal CRB2 antibody was raised against a 17 amino acid peptide near the center of human CRB2. The immunogen is located within amino acids 460 - 510 of CRB2.

Isotype: IgG

Application Details

Application Notes: CRB2 antibody can be used for detection of CRB2 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: CRB2 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: CRB2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for CRB2

Target: CRB2 (Crumbs Homolog 2 (CRB2))

Alternative Name: CRB2 (CRB2 Products)

Synonyms: 5930402A21 antibody, BC043114 antibody, RGD1309368 antibody, crb2 antibody, crumbs family member 2 antibody, crumbs 2, cell polarity complex component antibody, crumbs family member 2a antibody, Crb2 antibody, CRB2 antibody, crb2a antibody

Background: CRB2 Antibody: CRB2 (Crumbs homolog 2), like its homologs CRB1 and CRB3, is similar to the Drosophila crumbs protein and is expressed in retina, brain and kidney. Along with other proteins, the Crumbs proteins form a complex that help set up cell polarity in developing neuroepithelial cells. At the onset of neural specification, embryonic stem cells (ESCs) upregulate CRB2, which then localizes apically in neural rosettes. Gain- and loss-of-function studies of CRB2 have shown that CRB2 is essential for the stabilization of other polarity proteins. Unlike CRB1, mutations in CRB2 do not appear to play a role in retinitis pigmentosa or in Leber congenital amaurosis.

Molecular Weight: Predicted: 105, 129, and 141 kDa

Observed: 103, 128 kDa

Gene ID: 286204

NCBI Accession: NP_775960

UniProt: Q5IJ48

Pathways: Notch Signaling