HRAS antibody (C-Term)

Catalog No. ABIN6991870

This Rabbit Polyclonal antibody specifically detects HRAS in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HRAS antibody (C-Term) (ABIN6991870)

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRAS antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HRAS Antibody

Binding Specificity: C-Term

Specificity: N-RAS antibody is predicted to not cross-react with H-RAS

Purification: N-RAS Antibody is affinity chromatography purified via peptide column.

Immunogen: N-RAS antibody was raised against a 15 amino acid peptide near the carboxy terminus of human N-RAS. The immunogen is located within the last 50 amino acids of N-RAS.

Isotype: IgG

Application Details

Application Notes: N-RAS antibody can be used for detection of N-RAS by Western blot at 1 and 2 μ,g/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: N-RAS Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: N-RAS antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for HRAS

Target: HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name: N-RAS

Background: N-RAS Antibody: Activating mutations and overexpression of classical Ras subfamily members (N-RAS, H-RAS, and K-RAS) have been widely investigated as key events in the development of human cancers. The N-RAS protein shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. N-RAS, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.

Molecular Weight: Predicted: 22 kDa

Observed: 22 kDa

Gene ID: 4893

NCBI Accession: NP_002515

UniProt: P01111

Pathways: p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling