PRAS40 antibody (C-Term)

Catalog No. ABIN6991875

Product Details anti-PRAS40 Antibody

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Binding Specificity: AA 190-240, C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAS40 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: AKT1S1 antibody is human, mouse and rat reactive. At least three isoforms of AKT1S1 are known to exist.

Purification: AKT1S1 Antibody is affinity chromatography purified via peptide column.

Immunogen: AKT1S1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AKT1S1. The immunogen is located within amino acids 190 - 240 of AKT1S1.

Isotype: IgG

Application Details

Application Notes: AKT1S1 antibody can be used for detection of AKT1S1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: AKT1S1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: AKT1S1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name: AKT1S1 (AKT1S1 Products)

Synonyms: MGC81452 antibody, lobe antibody, pras40 antibody, fb34a04 antibody, wu:fb34a04 antibody, Lobe antibody, PRAS40 antibody, 1110012J22Rik antibody, AI227026 antibody, Lobel antibody, AKT1 substrate 1 (proline rich) S homeolog antibody, AKT1 substrate 1 antibody, AKT1 substrate 1 (proline rich) antibody, AKT1 substrate 1 (proline-rich) antibody, akt1s1.S antibody, AKT1S1 antibody, akt1s1 antibody, Akt1s1 antibody

Background: AKT1S1 Antibody: The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia (1). AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy (2). Treatment with the insulin-like growth factor-1 (IGF1) can indusce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells (3).

Molecular Weight: Predicted: 30 kDa

Observed: 28 kDa

Gene ID: 84335

NCBI Accession: NP_115751

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect