NCBP1 antibody (AA 420-470)

Catalog No. ABIN6991877

The Rabbit Polyclonal anti-NCBP1 antibody has been validated for WB, ELISA and ICC. It is suitable to detect NCBP1 in samples from Human, Mouse and Rat.

Quick Overview for NCBP1 antibody (AA 420-470) (ABIN6991877)

Target: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-NCBP1 Antibody

Binding Specificity: AA 420-470

Specificity: NCBP1 antibody is human, mouse and rat reactive. At least two isoforms of NCBP1 are known to exist, this antibody will detect both isoforms of NCBP1.

Purification: NCBP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: NCBP1 antibody was raised against a 17 amino acid peptide near the center of human NCBP1. The immunogen is located within amino acids 420 - 470 of NCBP1.

Isotype: IgG

Application Details

Application Notes: NCBP1 antibody can be used for detection of NCBP1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: NCBP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: NCBP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for NCBP1

Target: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Alternative Name: NCBP1

Background: NCBP1 Antibody: NCBP1, also known as CBP80, is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. NCBP1 promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay (1,2). Recent evidence has shown that cellular-cap-binding proteins such as NCBP1 associate with influenza virus mRNAs, suggesting that these viral mRNAs may follow the normal cellular pathways for splicing, nuclear export, and translation (3).

Molecular Weight: Predicted: 87 kDa

Observed: 87 kDa

Gene ID: 4686

NCBI Accession: NP_002477

UniProt: Q09161

Pathways: Ribonucleoprotein Complex Subunit Organization, Photoperiodism, Methionine Biosynthetic Process