LC3B antibody (N-Term)

Catalog No. ABIN6991932

Product Details anti-LC3B Antibody

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: MAP1LC3B antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3B are known to exist. MAP1LC3B antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3C

Purification: MAP1LC3B antibody is affinity chromatography purified via peptide column.

Immunogen: MAP1LC3B antibody was raised against an 18 amino acid peptide near the amino terminus of human MAP1LC3B. The immunogen is located within the first 50 amino acids of MAP1LC3B.

Isotype: IgG

Application Details

Application Notes: MAP1LC3B antibody can be used for detection of MAP1LC3B by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: MAP1LC3B antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: MAP1LC3B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: MAP1LC3B (MAP1LC3B Products)

Synonyms: ATG8F antibody, LC3B antibody, MAP1A/1BLC3 antibody, MAP1LC3B-a antibody, Map1lc3 antibody, Mpl3 antibody, zbs559 antibody, 1010001C15Rik antibody, Atg8 antibody, LC3b antibody, zgc:56434 antibody, wu:fb60g11 antibody, map1lc3b antibody, MGC76283 antibody, MAP1LC3B antibody, microtubule associated protein 1 light chain 3 beta antibody, microtubule-associated protein 1 light chain 3 beta antibody, microtubule associated protein 1 light chain 3 beta L homeolog antibody, MAP1LC3B antibody, Map1lc3b antibody, map1lc3b antibody, map1lc3b.L antibody

Background: Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3B belongs to the MAP1 LC3 family and it includes 3 different light chains, LC1, LC2 and LC3 (2). MAP1LC3B is involved in formation of autophagosomal vacuoles (autophagosomes) (3). It is most abundant in heart, brain, skeletal muscle and testis. MAP1LC3B is essential for autophagy and associated to the autophagosome membranes after processing (4).

Molecular Weight: Predicted: 14 kDa

Observed: 14 kDa

Gene ID: 81631

NCBI Accession: NP_073729

UniProt: Q9GZQ8

Pathways: Autophagy