TESPA1 antibody (N-Term)

Catalog No. ABIN6991940

Product Details anti-TESPA1 Antibody

Target: TESPA1 (Thymocyte Expressed, Positive Selection Associated 1 (TESPA1))

Binding Specificity: AA 20-70, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TESPA1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: TESPA1 antibody is human specific. At least three isoforms TESPA1 are known to exist.

Purification: TESPA1 antibody is affinity chromatography purified via peptide column.

Immunogen: TESPA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human TESPA1. The immunogen is located within amino acids 20 - 70 of TESPA1.

Isotype: IgG

Application Details

Application Notes: TESPA1 antibody can be used for detection of TESPA1 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TESPA1 antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TESPA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for TESPA1

Target: TESPA1 (Thymocyte Expressed, Positive Selection Associated 1 (TESPA1))

Alternative Name: TESPA1 (TESPA1 Products)

Synonyms: KIAA0748 antibody, thymocyte expressed, positive selection associated 1 antibody, TESPA1 antibody

Background: TESPA1 is a critical component of the TCR signalosome and is essential for T cell selection and maturation through the regulation of TCR signaling during T cell development (1). TESPA1 encodes a protein of 458 amino acids and is highly conserved among vertebrate species (2). TESPA1 expression was tightly regulated during T cell development, with highest expression at the DP stage (3). TESPA1 deficiency resulted in failure of positive selection and substantially fewer of CD4+ and CD8+ SP cells in the thymus (1,4).

Molecular Weight: Predicted: 57 kDa

Observed: 60 kDa

Gene ID: 9840

NCBI Accession: NP_001129502

UniProt: A2RU30