Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
DNM1L antibody is human and mouse reactive. At least four isoforms of DNM1L are known to exist, this antibody will detect the two longest isoforms.
Purification
DNM1L antibody is affinity chromatography purified via peptide column.
Immunogen
DNM1L antibody was raised against a 19 amino acid peptide near the center of human DNM1L. The immunogen is located within amino acids 530 - 580 of DNM1L.
DNM1L antibody can be used for detection of DNM1L by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
DNM1L antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
DNM1L antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
The Dynamin-1-like protein (DNM1L) is a member of the dynamin superfamily of GTPases (1). DNM1L mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis (2). Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF) (3).