DEPTOR antibody (AA 220-270)

Catalog No. ABIN6992003

Product Details anti-DEPTOR Antibody

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Binding Specificity: AA 220-270

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEPTOR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: DEPTOR antibody is human, mouse and rat reactive. At least three isoforms of DEPTOR are known to exist, this antibody will detect all three isoforms.

Purification: DEPTOR antibody is affinity chromatography purified via peptide column.

Immunogen: DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR. The immunogen is located within amino acids 220 - 270 of DEPTOR.

Isotype: IgG

Application Details

Application Notes: DEPTOR antibody can be used for detection of DEPTOR by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: DEPTOR Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: DEPTOR antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for DEPTOR

Target: DEPTOR (DEP Domain Containing mTOR-Interacting Protein (DEPTOR))

Alternative Name: DEPTO (DEPTOR Products)

Synonyms: DEP.6 antibody, DEPDC6 antibody, 4731402B04Rik antibody, 9130412E02Rik antibody, D15Ertd336e antibody, D15Ertd597e antibody, Depdc6 antibody, Depdc6-001 antibody, Depdc6-002 antibody, R75183 antibody, mKIAA4200 antibody, RGD1561030 antibody, RAPGEF4 antibody, DEP domain containing MTOR interacting protein antibody, DEP domain containing MTOR-interacting protein antibody, DEPTOR antibody, Deptor antibody

Background: DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).

Molecular Weight: Predicted: 45 kDa

Observed: 42 kDa

Gene ID: 64798

NCBI Accession: NP_073620

UniProt: Q8TB45

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Cell Size, BCR Signaling, Warburg Effect