Vinculin antibody is human, mouse and rat specific. At least three isoforms of Vinculin are known to exist.
Purification
Vinculin Antibody is affinity chromatography purified via peptide column.
Immunogen
Anti-Vinculin antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Vinculin. The immunogen is located within amino acids 1060-1110 of Vinculin.
VCL
Reactivity: Human
WB, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1-2 μ,g/mL, IHC: 5 μ,g/mL, IF: 20 μ,g/mL.
Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in rat samples, Immunofluorescence in rat samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Vinculin Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
Vinculin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).