Nephrin antibody (AA 1100-1150)

Catalog No. ABIN6992074

Product Details anti-Nephrin Antibody

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Binding Specificity: AA 1100-1150

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nephrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: Nephrin antibody is affinity chromatography purified via peptide column.

Immunogen: Anti-Nephrin antibody was raised against a peptide corresponding to 15 amino acids near the center of human Nephrin. The immunogen is located within amino acids 1100-1150 of Nephrin.

Isotype: IgG

Application Details

Application Notes: WB: 1-2 μ,g/mL, IHC: 5 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human and mouse samples, Immunohistochemistry in human samples, Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Nephrin antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: Nephrin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for Nephrin

Target: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Alternative Name: Nephrin (NPHS1 Products)

Synonyms: NPHS1 antibody, nphs1l antibody, nphs1 antibody, CNF antibody, NPHN antibody, nephrin antibody, NephrinB antibody, NPHS1, nephrin antibody, nephrosis 1, congenital, Finnish type (nephrin) antibody, NPHS1 nephrin S homeolog antibody, nephrin antibody, NPHS1 nephrin antibody, nephrosis 1, nephrin antibody, NPHS1 antibody, nphs1 antibody, nphs1.S antibody, LOC5579127 antibody, Nphs1 antibody

Background: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth (1). Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm (2,3).

Molecular Weight: Predicted: 125, 137 kDa

Observed: 125 kDa

Gene ID: 4648

NCBI Accession: NP_004637

UniProt: O60500

Pathways: Regulation of Actin Filament Polymerization, Skeletal Muscle Fiber Development