GAD65 antibody

Catalog No. ABIN7426745

The Mouse Monoclonal anti-GAD65 antibody (Clone 6-1#) (ABIN7426745) specifically detects GAD65 in WB, IHC, IP and ICC. The antibody is reactive with Rat samples.

Quick Overview for GAD65 antibody (ABIN7426745)

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GAD65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 6-1#

Product Details anti-GAD65 Antibody

Purpose: Monoclonal Antibody to Glutamate Decarboxylase 2 (GAD2)

Specificity: The antibody is a mouse monoclonal antibody raised against GAD2. It has been selected for its ability to recognize GAD2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Glutamate Decarboxylase 2 (GAD2) corresdonding to N-terminal His Tag

Isotype: IgG2a kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:500-2000Immunohistochemistry: 5-20 μg/mL,1:50-200Immunocytochemistry: 5-20 μg/mL,1:50-200Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Alternative Name: GAD2

Background: GAD65, Glutamate Decarboxylase 2, Acid, Pancreatic Islets And Brain,65 kDa, 65 kDa glutamic acid decarboxylase, Glutamate decarboxylase 65 kDa isoform

UniProt: Q05683