BRAF antibody (AA 457-717)

Catalog No. ABIN7427177

The Mouse Monoclonal anti-BRAF antibody (Clone C5) (ABIN7427177) specifically detects BRAF in WB, IHC, ICC and IP. The antibody is reactive with Human samples.

Quick Overview for BRAF antibody (AA 457-717) (ABIN7427177)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: C5

Product Details anti-BRAF Antibody

Binding Specificity: AA 457-717

Purpose: Monoclonal Antibody to B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF)

Specificity: The antibody is a mouse monoclonal antibody raised against BRAF. It has been selected for its ability to recognize BRAF in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant B-Raf Proto Oncogene Serine/Threonine Protein Kinase (BRAF) corresdonding to Ile457~Leu717 with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.47 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF

Background: B-raf 1, BRAF1, RAFB1, V-Raf Murine Sarcoma Viral Oncogene Homolog B1

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy