GCKR antibody (AA 90-286)

Catalog No. ABIN7442180

This anti-GCKR antibody is a Rabbit Polyclonal antibody detecting GCKR in WB, IHC, IP and ICC. Suitable for Rat.

Quick Overview for GCKR antibody (AA 90-286) (ABIN7442180)

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCKR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-GCKR Antibody

Binding Specificity: AA 90-286

Purpose: Polyclonal Antibody to Glucokinase Regulatory Protein (GKRP)

Specificity: The antibody is a rabbit polyclonal antibody raised against GKRP. It has been selected for its ability to recognize GKRP in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Glucokinase Regulatory Protein (GKRP) corresdonding to Val90~Ala286 (Accession # Q07071) with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GCKR

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Alternative Name: GKRP

Background: GCKR, Glucokinase(hexokinase 4)regulator

UniProt: Q07071

Pathways: Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus