ATP4A antibody (AA 786-1014)
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- Target See all ATP4A Antibodies
- ATP4A (ATPase, H+/K+ Exchanging, alpha Polypeptide (ATP4A))
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Binding Specificity
- AA 786-1014
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ATP4A antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against ATP4a. It has been selected for its ability to recognize ATP4a in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Human, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) corresdonding to Pro786~Cys1014 (Accession # Q91WH7) with N-terminal His and GST Tag
- Isotype
- IgG
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- Application Notes
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Western blotting: 0.5-2 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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Crosstalk between Acidosis and Iron Metabolism: Data from In Vivo Studies." in: Metabolites, Vol. 12, Issue 2, (2022) (PubMed).
: "
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Crosstalk between Acidosis and Iron Metabolism: Data from In Vivo Studies." in: Metabolites, Vol. 12, Issue 2, (2022) (PubMed).
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- Target
- ATP4A (ATPase, H+/K+ Exchanging, alpha Polypeptide (ATP4A))
- Alternative Name
- ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4A Products)
- Synonyms
- ATP6A antibody, Hka antibody, Hkatpc antibody, ATPase H+/K+ transporting alpha subunit antibody, ATPase H+/K+ transporting alpha subunit L homeolog antibody, ATPase, H+/K+ exchanging, gastric, alpha polypeptide antibody, ATP4A antibody, Atp4a antibody, atp4a.L antibody
- Background
- Gastric H,K-ATPase Alpha Subunit, H(+)-K(+)-ATPase Alpha Subunit, Proton Pump, Potassium-transporting ATPase alpha chain 1
- Pathways
- Proton Transport, Ribonucleoside Biosynthetic Process
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