DYKDDDDK Tag antibody

Catalog No. ABIN7005787

Product Details anti-DYKDDDDK Tag Antibody

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Characteristics: Tag Antibody

Purification: Protein A purification

Immunogen: Synthetic Peptide

Clone: 4A2

Isotype: IgG

Application Details

Application Notes: WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Alternative Name: Flag-Tag (DYKDDDDK Tag Products)

Target Type: Tag

Background: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies.