EPH Receptor B6 antibody (Internal Region)

Catalog No. ABIN7013872

The Goat Polyclonal anti-EPH Receptor B6 antibody has been validated for ELISA, IHC, IF and FACS. It is suitable to detect EPH Receptor B6 in samples from Human.

Quick Overview for EPH Receptor B6 antibody (Internal Region) (ABIN7013872)

Target: EPH Receptor B6 (EPHB6)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EPH Receptor B6 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-EPH Receptor B6 Antibody

Binding Specificity: Internal Region

Purpose: EPHB6

Sequence: EVIAHGKHTT SSD

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine and Brain (Cerebellum). Recommended concentration: 3.75 μg/mL.

Western Blot: Preliminary testing showed a band at approx 80 kDa in U251 cell lysate and approx.75 kDa in Human Pancreas lysate after 1-2 μg/mL antibody staining (calculated MW of 79.8 kDa according to NP_001267723.2). Primary incubation 1 hour at room tem

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended c

Restrictions: For Research Use only

Handling

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EPH Receptor B6

Target: EPH Receptor B6 (EPHB6)

Alternative Name: EPHB6

Background: EPHB6, EPH receptor B6, HEP, MGC129910, MGC129911, ephrin receptor EphB6

Gene ID: 2051, 13848, 312275

Pathways: RTK Signaling, Hormone Transport