BDNF antibody (Intracellular)
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- Target See all BDNF Antibodies
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
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Binding Specificity
- AA 166-178, Intracellular
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Reactivity
- Human, Rat, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This BDNF antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Cross-Reactivity (Details)
- The antibody is specific for BDNF, it does not crossreact with NGF, NT-3 or NT-4.
- Characteristics
- Anti-BDNF Antibody (ABIN7042970, ABIN7044761 and ABIN7044762)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize BDNF from rat, human, and mouse samples. The antibody is specific for BDNF, it does not crossreact with NGF, NT-3 or NT-4.
- Purification
- Affinity purified on immobilized antigen.
- Immunogen
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)VLEKVPVSKQLK, corresponding to amino acid residues 166-178 of human BDNF (precursor)
- Isotype
- IgG
- Top Product
- Discover our top product BDNF Primary Antibody
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
- Concentration
- 0.72 mg/mL
- Buffer
- Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1 % BSA, 0.05 % Sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Target
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
- Alternative Name
- BDNF (BDNF Products)
- Synonyms
- ANON2 antibody, BULN2 antibody, bdnf-A antibody, BDNF antibody, bdnf antibody, brain derived neurotrophic factor antibody, brain-derived neurotrophic factor antibody, brain-derived neurotrophic factor L homeolog antibody, brain-derived neurotrophic factor S homeolog antibody, BDNF antibody, Bdnf antibody, bdnf antibody, bdnf.L antibody, bdnf.S antibody
- Background
- Alternative names: Brain Derived Neurotrophic Factor, Brain Derived Neurotrophic Factor
- Gene ID
- 627
- NCBI Accession
- NM_170735
- UniProt
- P23560
- Pathways
- RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
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