TOP3A antibody

Catalog No. ABIN7075939

Product Details anti-TOP3A Antibody

Target: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP3A antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human

Purification: Affinity purification

Immunogen: KLH conjugated Synthetic peptide corresponding to Mouse Topoisomerase III alpha

Application Details

Application Notes: WB (H) 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for TOP3A

Target: TOP3A (Topoisomerase (DNA) III alpha (TOP3A))

Alternative Name: Topoisomerase III alpha (TOP3A Products)

Synonyms: TOP3 antibody, DNA topoisomerase III alpha antibody, topoisomerase (DNA) III alpha antibody, Top3a antibody, TOP3A antibody

Background: DNA topoisomerase 3-alpha (TOP3A) is an essential component of the RMI complex, a complex involves in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. It releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils.

Molecular Weight: 112 kDa

Gene ID: 21975

NCBI Accession: NP_033436

UniProt: O70157

Pathways: DNA Damage Repair