FAP antibody (AA 300-400)

Catalog No. ABIN7091105

Product Details anti-FAP Antibody

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Binding Specificity: AA 300-400

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAP antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FAPA

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: FAPA (FAP Products)

Synonyms: fap-A antibody, gene 13 antibody, FAP antibody, fapa antibody, dppiv antibody, seprase antibody, DPPIV antibody, FAPA antibody, fibroblast activation protein antibody, fibroblast activation protein alpha L homeolog antibody, fibroblast activation protein alpha antibody, fibroblast activation protein, alpha antibody, prolyl endopeptidase FAP antibody, Fap antibody, fap.L antibody, FAP antibody, fap antibody, LOC100547273 antibody

Background:

Synonyms: Fibroblast activation protein, alpha, 170 kDa melanoma membrane bound gelatinase, 170 kDa melanoma membrane-bound gelatinase, DPPIV, FAP, Fibroblast activation protein alpha, Integral membrane serine protease, SEPR_HUMAN, Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, FAPalpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, SIMP, Surface-expressed protease, Seprase, Antiplasmin-cleaving enzyme FAP, soluble form, APCE.

Background: Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2. Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vibronectin, tenascin, laminin, fibronectin, fibrin or casein. Have also dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB) (PubMed:21314817). The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Plays a role in tissue remodeling during development and wound healing. Participates in the cell invasiveness towards the ECM in malignant melanoma cancers. Enhances tumor growth progression by increasing angiogenesis, collagen fiber degradation and apoptosis and by reducing antitumor response of the immune system. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.

Gene ID: 2191

UniProt: Q12884

Pathways: Tube Formation