GAD65 antibody

Catalog No. ABIN7114595

The Rabbit Polyclonal anti-GAD65 antibody is suitable to detect GAD65 in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for GAD65 antibody (ABIN7114595)

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAD65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-GAD65 Antibody

Purpose: GAD2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)

Isotype: IgG

Application Details

Application Notes: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:10 - 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for GAD65

Target: GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))

Alternative Name: GAD2

Background:

Synonyms: Glutamate decarboxylase 2|65 kDa glutamic acid decarboxylase (GAD-65)|Glutamate decarboxylase 65 kDa isoform|GAD2|GAD65

Background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Molecular Weight: 70 kDa

Gene ID: 2572

UniProt: Q05329