PAD4 antibody

Catalog No. ABIN7117295

Product Details anti-PAD4 Antibody

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: peptidyl arginine deiminase, type IV

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:100-1:500, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for PAD4

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Alternative Name: PADI4 (PADI4 Products)

Synonyms: PAD antibody, PAD4 antibody, PADI5 antibody, PDI4 antibody, PDI5 antibody, Pad4 antibody, Pdi4 antibody, peptidyl arginine deiminase 4 antibody, peptidyl arginine deiminase, type IV antibody, PADI4 antibody, Padi4 antibody

Background: Synonyms:PAD4, PADI5, PDI5 Background:Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54'(to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26'(to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3'(to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1(H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.

Molecular Weight: 74 kDa

Gene ID: 23569

UniProt: Q9UM07