TJP1 antibody

Catalog No. ABIN7119969

Product Details anti-TJP1 Antibody

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS)

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: tight junction protein 1(zona occludens 1)

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IP: 1:200-1:2000, IHC: 1:50-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for TJP1

Target: TJP1 (Tight Junction Protein 1 (TJP1))

Alternative Name: ZO-1 (TJP1 Products)

Synonyms: ZO-1 antibody, ZO1 antibody, zo1 antibody, tight junction protein 1 antibody, tight junction protein ZO-1 antibody, TJP1 antibody, Tjp1 antibody, LOC396567 antibody

Background: Synonyms:Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, ZO-1, Zona occludens protein 1, Zonula occludens protein 1 Background:Tight junction(or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium(PMID: 20066090). ZO-1(also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells(PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin(PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs(MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation(PMID: 22782886).

Molecular Weight: 230kd

Gene ID: 7082

UniProt: Q07157

Pathways: Carbohydrate Homeostasis, Cell-Cell Junction Organization